报告题目: Phospholipids in Atlantic cod larvae rearing
报告人: 李克帅 博士🫄🏽，挪威科技大学(NTNU) Research Fellow
主持人: 杜震宇 教授
报告时间: 2017年4月18日 9:50-10:30 （周二上午）
报告地点: 天美娱乐534报告厅
报告人简介：李克帅博士是挪威科技大学的Research Fellow, 主要从事鱼类脂类营养研究，其重点在于稚幼鱼磷脂代谢的研究。李博士已在鱼类营养代谢生理主流期刊上发表论文十余篇，是当前鱼类营养领域较为活跃的青年学者⛩。
报告摘要💁🏿：The importance of dietary PL (phospholipids) for fish larvae has been well demonstrated. Recent studies have shown that fish larvae require not only a certain quantitative amount of dietary PL in their feed, but they also depend on the quality of the dietary PL, and their DHA content is particularly important for normal growth and functional development. The most commonly used live feeds in aquaculture, rotifers (Brachionus sp.) and brine shrimp (Artemia sp.), do not contain adequate amounts of DHA in their PL. Knowing now that fish larvae may have specific requirements to their dietary PL composition, there is an emerging need to learn more on how PL of live feed organisms can be efficiently enriched. Our preliminary results have suggested that the DHA content in the main PL classes of rotifers could be affected by fatty acids such as 16:0 and 18:1n9, but it needs further investigation for final confirmation.
The mechanism behind the PL requirement of fish larvae is not well understood and the main hypothesis is that fish larvae have a limited ability to biosynthesize PL de novo, which is necessary for lipoprotein transport from the enterocytes. The full genome of Atlantic cod (Gadus morhua) has been sequenced recently, and will contribute to improve the fundamental biological knowledge of the species. The overall objective of our studies has been to obtain more knowledge of ontogenesis of PL synthesis capability of early stages of cod. Transcriptome analysis of larvae in different stages was carried out using a recently developed Atlantic cod microarray. Moreover, due to the non-transcriptional regulation of PL biosynthesis, a tube feeding method was used delivering labelled lipid precursors to cod larvae to evaluate their capacity of PL synthesis. The results showed no major effect of development on the expression of key genes of PL biosynthesis, suggesting no genetic constrain in early developmental stages. The 30 days post hatch larvae showed relatively high biosynthesis ability of PL compared to neutral lipids. Our overall data suggested that besides the possible limited de novo PL synthesis ability in the intestine, other metabolic constraints should also be considered.
